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Samonella typhi ,S.paratyphi A , S.paratyphi B -etiological agent of Typhoid and Paratyphoid Fever

Samonella typhi ,S.paratyphi A , S.paratyphi B -etiological agent of typhoid and paratyphoid fever

Classification of Salmonella genus
Salmonella genus classified into 2 species
1.S.enterica- has been divided into 6 subspecies.(serotypes)
- cause infection in men and animals.
2.S.bongori- has been subdivided into6 subspecies. (serotypes)
- Cause infection in animals only.
Family : Enterobactericeae
Tribe : Escherichiae
Genus : Salmonella
Species : Salmonella enterica
Subspecies : a) Salmonella typhi (man)
b) Salmonella paratyphi A (man)
c) Salmonella paratyphi B (man, animals)

Salmonella morphologically short, gram negative rods, non-sporing, non-capsulated but flagellated. These microorganism are mesophiles (37 temperature growth), facultative anaerobs, can grow in simple media.

Cultural properties

Salmonella can grow in MPA / MPB
Selective media to cultivate Salmonella are:

•Bile broth (liquid medium)
•MacConkey media
•Salmonella-Shigella agar (SSA)
•Eosin Methylene Blue (EMB)
•Bismuth-containing agar
•Rapaport’s medium (this medium consist of 10%bile broth, 1%glucose, indicator)


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